Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis.
SLAS Discov
; 28(2): 13-19, 2023 03.
Article
em En
| MEDLINE
| ID: mdl-36116760
ABSTRACT
Three dimensional models of cell culture enables researchers to recreate aspects of tumour biology not replicated by traditional two dimensional techniques. Here we describe a protocol to enable automated high throughput phenotypic profiling across panels of patient derived glioma stem cell spheroid models. We demonstrate the use of both live/dead cell end-points and monitor the dynamic changes in the cell cycle using cell lines expressing the FUCCI cell cycle reporter. Together, these assays provide additional insight into the mechanism of action of compound treatments over traditional cell viability assay endpoints.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esferoides Celulares
/
Glioma
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
SLAS Discov
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Reino Unido