Aerotaxis Assay in Caenorhabditis elegans to Study Behavioral Plasticity.
Bio Protoc
; 12(16)2022 Aug 20.
Article
em En
| MEDLINE
| ID: mdl-36199707
ABSTRACT
C. elegans shows robust and reproducible behavioral responses to oxygen. Specifically, worms prefer O 2 levels of 5-10% and avoid too high or too low O 2 . Their O 2 preference is not fixed but shows plasticity depending on experience, context, or genetic background. We recently showed that this experience-dependent plasticity declines with age, providing a useful behavioral readout for studying the mechanisms of age-related decline of neural plasticity. Here, we describe a technique to visualize behavioral O 2 preference and its plasticity in C. elegans , by creating spatial gradients of [O 2 ] in a microfluidic polydimethylsiloxane (PDMS) chamber and recording the resulting spatial distribution of the animals.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Reino Unido