[Regulation of PARP-1 deficiency on epidermal growth factor receptor during lung injury of mice induced by benzo [a] pyrene inhalation exposure].
Wei Sheng Yan Jiu
; 51(5): 787-790, 2022 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-36222041
ABSTRACT
OBJECTIVE:
To investigate the regulation of PARP-1 deficiency on epidermal growth factor receptor(EGFR) during lung injury of mice induced by benzo[a]pyrene(B[a]P) inhalation exposure.METHODS:
PARP-1 knockout mice(PARP-1~(-/-)) and WT mice were selected as the object, and which were randomly assigned into either an intervention or a control group(n=40, half male and half female). The intervention group were individually treated with 10.0 µg/m~(3 )B[a]P for 180 days by dynamic inhalation exposure(6 h per day and 5 days per week), and the control group was given the solvent dimethyl sulfoxide(DMSO) during the same period. The expression of EGFR in lung tissues of animals were examined by RT-PCR, Western blot and immunofluorescence.RESULTS:
In WT mice, the intervention manifested significant increase expression of EGFR in lung tissue, but no changes were found in the control. In PARP-1~(-/-) mice, the intervention manifested significant inhibition expression of EGFR, but the control group exhibited no changes.CONCLUSION:
PARP-1 deficiency suppresses the abnormal activation of EGFR during lung injury of mice induced by B[a]P inhalation exposure.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Benzo(a)pireno
/
Lesão Pulmonar
Limite:
Animals
Idioma:
Zh
Revista:
Wei Sheng Yan Jiu
Assunto da revista:
SAUDE PUBLICA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China