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Exosomal TAR DNA binding protein 43 profile in canine model of amyotrophic lateral sclerosis: a preliminary study in developing blood-based biomarker for neurodegenerative diseases.
Pfeiffer, Penelope; Coates, Joan R; Esqueda, Yajaira M; Kennedy, Andrew; Getchell, Kyleigh; McLenon, Myra; Kosa, Edina; Agbas, Abdulbaki.
Afiliação
  • Pfeiffer P; Kansas City University, Kansas City, MO, USA.
  • Coates JR; University of Missouri-Columbia, Columbia, MO, USA.
  • Esqueda YM; Kansas City University, Kansas City, MO, USA.
  • Kennedy A; Kansas City University, Kansas City, MO, USA.
  • Getchell K; Kansas City University, Kansas City, MO, USA.
  • McLenon M; Kansas City University, Kansas City, MO, USA.
  • Kosa E; Kansas City University, Kansas City, MO, USA.
  • Agbas A; Kansas City University, Kansas City, MO, USA.
Ann Med ; 55(1): 34-41, 2023 12.
Article em En | MEDLINE | ID: mdl-36495266
OBJECTIVE: Blood-based biomarkers provide a crucial information in the progress of neurodegenerative diseases with a minimally invasive sampling method. Validated blood-based biomarker application in people with amyotrophic lateral sclerosis would derive numerous benefits. Canine degenerative myelopathy is a naturally occurring animal disease model to study the biology of human amyotrophic lateral sclerosis. Serum derived exosomes are potential carriers for cell-specific cargoes making them ideal venue to study biomarkers for a variety of diseases and biological processes. This study assessed the exosomal proteins that may be assigned as surrogate biomarker that may reflect biochemical changes in the central nervous system. METHODS: Exosomes were isolated from canine serum using commercial exosome isolation reagents. Exosomes target proteins contents were analyzed by the Western blotting method. RESULTS: The profiles of potential biomarker candidates in spinal cord homogenate and that of serum-derived exosomes were found elevated in dogs with degenerative myelopathy as compared to control subjects. CONCLUSIONS: Serum-derived exosomal biomolecules can serve as surrogate biomarkers in neurodegenerative diseases.KEY MESSAGESA canine with degenerative myelopathy can serve as a model animal to study human amyotrophic lateral sclerosis.Serum-derived exosomes contain Transactive Response DNA Binding Protein 43 (TDP-43), a potential biomarker candidate.The levels of spinal cord TDP-43 proteins and that of serum-derived exosomes exhibited similar profiling. Therefore, serum derived exosomes may be used as a venue for establishing blood-based biomarkers for neurodegenerative diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Neurodegenerativas / Exossomos / Esclerose Lateral Amiotrófica Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Ann Med Assunto da revista: MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Neurodegenerativas / Exossomos / Esclerose Lateral Amiotrófica Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Ann Med Assunto da revista: MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
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