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Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter.
Sergeeva, M V; Pulkina, A A; Romanovskaya-Romanko, E A; Mustafaeva, A S; Egorov, A Yu; Stukova, M A.
Afiliação
  • Sergeeva MV; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, Russia.
  • Pulkina AA; Peter the Great St. Petersburg Polytechnic University, 195251 St. Petersburg, Russia.
  • Romanovskaya-Romanko EA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, Russia.
  • Mustafaeva AS; Peter the Great St. Petersburg Polytechnic University, 195251 St. Petersburg, Russia.
  • Egorov AY; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, Russia.
  • Stukova MA; St. Petersburg State Institute of Technology, 190013 St. Petersburg, Russia.
Appl Biochem Microbiol ; 58(7): 878-886, 2022.
Article em En | MEDLINE | ID: mdl-36532244
Influenza viruses cause acute respiratory infections, especially in the autumn-winter period. They are characterized by a high mutation frequency and cause annual seasonal epidemics. The detection of antibodies that neutralize the virus is an important criterion in the assessment of population immunity and the influenza vaccine effectiveness. In this study, a method for determining the titer of virus-neutralizing antibodies in blood serum has been developed. A new test called the luciferase neutralization assay uses a bioluminescent signal for detection. The assay is based on engineered influenza reporter viruses with various surface antigens and a nanoluciferase reporter protein in the NS1 reading frame. Using the developed method, we studied paired sera of volunteers obtained before and after vaccination. The proposed assay was compared with the conventional antibody assessment methods (microneutralization and hemagglutination inhibition assay); a high degree of correlation was observed. At the same time, the use of the luciferase neutralization assay made it possible to reduce the time required for the analysis and to simplify the detection procedure. Supplementary Information: The online version contains supplementary material available at 10.1134/S0003683822070067.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Appl Biochem Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Federação Russa

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Appl Biochem Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Federação Russa
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