Clonogenic assay to measure bystander cytotoxicity of targeted alpha-particle therapy.
Methods Cell Biol
; 174: 137-149, 2023.
Article
em En
| MEDLINE
| ID: mdl-36710047
ABSTRACT
Radiation therapy induces targeted effects in the cells that are irradiated and also non-targeted effects (i.e. bystander effects) in non-irradiated cells that are close to or at short distance (<â¼1 mm) from irradiated cells. Bystander effects are mediated by intercellular communications and may result in cytotoxic and genotoxic modifications. Their occurrence and relative contribution to the irradiation outcome are influenced by several parameters among which the particle linear energy transfer seems to be prominent. Bystander effects were first observed after external radiation therapy, but have been described also following targeted radionuclide therapy. Therefore, we propose a method to investigate their occurrence in experimental conditions where cells are exposed to radiopharmaceuticals. In this approach, clonogenic cell death is the biological endpoint of the bystander effects caused by irradiation with alpha particles (a potent inducer of the bystander response).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Comunicação Celular
/
Partículas alfa
Idioma:
En
Revista:
Methods Cell Biol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
França