The effect of intentionally perforating the floor of the pulp chamber on pulpal healing after tooth replantation in mice.
J Oral Biosci
; 65(1): 31-39, 2023 03.
Article
em En
| MEDLINE
| ID: mdl-36737038
ABSTRACT
OBJECTIVES:
Shortening the root of a mouse molar prior to tooth replantation results in early revascularization in the pulp cavity and activation of the dental pulp quiescent stem cells. This study aimed to validate the effects of pulp chamber floor perforation on pulpal healing after tooth replantation as a strategy to promote early revascularization into the pulp.METHODS:
The maxillary first molars of three-week-old CrljCD1 mice were extracted and repositioned into the original socket the left teeth were immediately replanted (control group CG), whereas the floor of the pulp chamber of the right teeth was perforated with a tungsten carbide bur before tooth replantation (experimental group EG). The samples were collected from three days to eight weeks postoperatively. In addition to the TUNEL assay, immunohistochemistry for Nestin, CK14, and Ki-67 was conducted.RESULTS:
In the EG, early revascularization occurred with a decrease in apoptosis and an increase in cell proliferation, facilitating pulpal healing, compared with the CG. The rate of Nestin-positive perimeter in the distal root significantly increased on days 5 and 14 and the amount of Nestin-positive hard tissue increased on day 14. However, on day 7, the number of epithelial cell rests of Malassez in the EG significantly decreased, making the EG susceptible to ankylosis at the floor.CONCLUSIONS:
Intentionally perforating the floor of the pulp chamber provides a route for early revascularization, resulting in better pulpal healing after tooth replantation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anquilose Dental
/
Polpa Dentária
Limite:
Animals
Idioma:
En
Revista:
J Oral Biosci
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Japão