Preparation of Albatrellus ovinus ß-Glucan Microparticles with Dectin-1a Binding Properties.
ACS Appl Bio Mater
; 6(5): 1863-1872, 2023 05 15.
Article
em En
| MEDLINE
| ID: mdl-37075203
ABSTRACT
Fungal ß-glucans are compounds with the potential to activate the innate immune system, in part through binding to the receptor dectin-1. In the present study, small-scale methods for preparing dectin-1a binding microparticles from Albatrellus ovinus alkali-soluble ß-glucans were investigated. Mechanical milling was time-consuming and yielded large particles with wide size distributions. Precipitation was more successful the ß-glucan was dissolved in 1 M NaOH, diluted, and precipitated in 11 mol equiv HCl. This yielded particles in sizes ranging from 0.5-2 µm. The dectin-1a binding activity was determined using HEK-Blue reporter cells. The prepared particles were able to bind to dectin-1a to the same extent as baker's-yeast-derived ß-glucan particles. The precipitation method was convenient as a quick method for small-scale preparation of ß-glucan microparticle dispersions from mushroom ß-glucans.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-Glucanas
Idioma:
En
Revista:
ACS Appl Bio Mater
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Noruega