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Circulating miR-26b-5p and miR-451a as diagnostic biomarkers in medullary thyroid carcinoma patients.
Besharat, Z M; Trocchianesi, S; Verrienti, A; Ciampi, R; Cantara, S; Romei, C; Sabato, C; Noviello, T M R; Po, A; Citarella, A; Caruso, F P; Panariello, I; Gianno, F; Carpino, G; Gaudio, E; Chiacchiarini, M; Masuelli, L; Sponziello, M; Pecce, V; Ramone, T; Maino, F; Dotta, F; Ceccarelli, M; Pezzullo, L; Durante, C; Castagna, M G; Elisei, R; Ferretti, E.
Afiliação
  • Besharat ZM; Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy.
  • Trocchianesi S; Department of Molecular Medicine, Sapienza University of Rome, 00161, Rome, Italy.
  • Verrienti A; Department of Translational and Precision Medicine, Sapienza University of Rome, 00161, Rome, Italy.
  • Ciampi R; Endocrine Unit, Department of Clinical and Experimental Medicine, University of Pisa, 56126, Pisa, Italy.
  • Cantara S; Department of Medical, Surgical and Neurological Sciences, University of Siena, 53100, Siena, Italy.
  • Romei C; Endocrine Unit, Department of Clinical and Experimental Medicine, University of Pisa, 56126, Pisa, Italy.
  • Sabato C; Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy.
  • Noviello TMR; Biogem Scarl, Istituto di Ricerche Genetiche "Gaetano Salvatore", 83031, Ariano Irpino, Italy.
  • Po A; Department of Electrical Engineering and Information Technology, University of Naples Federico II, 80138, Naples, Italy.
  • Citarella A; Department of Molecular Medicine, Sapienza University of Rome, 00161, Rome, Italy.
  • Caruso FP; Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy.
  • Panariello I; Biogem Scarl, Istituto di Ricerche Genetiche "Gaetano Salvatore", 83031, Ariano Irpino, Italy.
  • Gianno F; Department of Electrical Engineering and Information Technology, University of Naples Federico II, 80138, Naples, Italy.
  • Carpino G; Thyroid Surgical Unit, IRCCS Fondazione G.Pascale, 80131, Naples, Italy.
  • Gaudio E; Department of Radiological, Oncological and Anatomo-Pathological Sciences, Sapienza University of Rome, 00161, Rome, Italy.
  • Chiacchiarini M; Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy.
  • Masuelli L; Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy.
  • Sponziello M; Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy.
  • Pecce V; Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy.
  • Ramone T; Department of Translational and Precision Medicine, Sapienza University of Rome, 00161, Rome, Italy.
  • Maino F; Department of Translational and Precision Medicine, Sapienza University of Rome, 00161, Rome, Italy.
  • Dotta F; Endocrine Unit, Department of Clinical and Experimental Medicine, University of Pisa, 56126, Pisa, Italy.
  • Ceccarelli M; Department of Medical, Surgical and Neurological Sciences, University of Siena, 53100, Siena, Italy.
  • Pezzullo L; Department of Medical, Surgical and Neurological Sciences, University of Siena, 53100, Siena, Italy.
  • Durante C; Tuscany Centre for Precision Medicine (CReMeP), 53100, Siena, Italy.
  • Castagna MG; Biogem Scarl, Istituto di Ricerche Genetiche "Gaetano Salvatore", 83031, Ariano Irpino, Italy.
  • Elisei R; Department of Electrical Engineering and Information Technology, University of Naples Federico II, 80138, Naples, Italy.
  • Ferretti E; Thyroid Surgical Unit, IRCCS Fondazione G.Pascale, 80131, Naples, Italy.
J Endocrinol Invest ; 46(12): 2583-2599, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37286863
ABSTRACT
PURPOSE/

METHODS:

The determination of tumour biomarkers is paramount to advancing personalized medicine, more so in rare tumours like medullary thyroid carcinoma (MTC), whose diagnosis is still challenging. The aim of this study was to identify non-invasive circulating biomarkers in MTC. To achieve this goal, paired MTC tissue and plasma extracellular vesicle samples were collected from multiple centres and microRNA (miRNA) expression levels were evaluated.

RESULTS:

The samples from a discovery cohort of 23 MTC patients were analysed using miRNA arrays. Lasso logistic regression analysis resulted in the identification of a set of circulating miRNAs as diagnostic biomarkers. Among them, miR-26b-5p and miR-451a, were highly expressed and their expression decreased during follow-up in disease-free patients in the discovery cohort. Circulating miR-26b-5p and miR-451a were validated using droplet digital PCR in a second independent cohort of 12 MTC patients.

CONCLUSION:

This study allowed the identification and validation of a signature of two circulating miRNAs, miR-26b-5p and miR-451a, in two independent cohorts reporting a significant diagnostic performance for MTC. The results of this study offer advancements in molecular diagnosis of MTC proposing a novel non-invasive tool to use in precision medicine.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Glândula Tireoide / MicroRNAs / MicroRNA Circulante Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: J Endocrinol Invest Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Itália
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Glândula Tireoide / MicroRNAs / MicroRNA Circulante Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: J Endocrinol Invest Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Itália