Expression and Purification of Inflammasome Sensor NOD-Like Receptor Protein-1 Using the Baculovirus-Insect Cell Expression System.
Methods Mol Biol
; 2691: 199-206, 2023.
Article
em En
| MEDLINE
| ID: mdl-37355547
ABSTRACT
Inflammasomes are innate immune sensing and signaling complexes critical for defense against pathogens and response to cellular stresses. A core component of inflammasomes is the sensor protein, which, upon sensing pathogen- or danger-associated molecular patterns (PAMPs or DAMPs), converts from inactive to active signaling platform for initiation of inflammatory signaling. A reliable source for the production and purification of recombinant inflammasome sensors is therefore invaluable for biochemical and structural characterizations, as well as drug screening for the development of therapeutics. Here, we describe an expression and purification protocol using the baculovirus-insect cell expression system to generate recombinant NLRP1, an important member of the NOD-like receptor (NLR) family of inflammasome sensors.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inflamassomos
/
Proteínas NLR
Tipo de estudo:
Guideline
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Austrália