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Spatially resolved single-cell translatomics at molecular resolution.
Zeng, Hu; Huang, Jiahao; Ren, Jingyi; Wang, Connie Kangni; Tang, Zefang; Zhou, Haowen; Zhou, Yiming; Shi, Hailing; Aditham, Abhishek; Sui, Xin; Chen, Hongyu; Lo, Jennifer A; Wang, Xiao.
Afiliação
  • Zeng H; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Huang J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Ren J; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Wang CK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Tang Z; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Zhou H; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Zhou Y; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Shi H; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Aditham A; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Sui X; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Chen H; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Lo JA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Wang X; Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Science ; 380(6652): eadd3067, 2023 06 30.
Article em En | MEDLINE | ID: mdl-37384709
ABSTRACT
The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 cells and revealing cell type-specific and brain region-specific translational regulation, including translation remodeling during oligodendrocyte maturation. Our method detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biossíntese de Proteínas / Encéfalo / RNA Mensageiro / Neuroglia / Mapeamento Cromossômico / Análise da Expressão Gênica de Célula Única / Neurônios Limite: Animals / Humans Idioma: En Revista: Science Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biossíntese de Proteínas / Encéfalo / RNA Mensageiro / Neuroglia / Mapeamento Cromossômico / Análise da Expressão Gênica de Célula Única / Neurônios Limite: Animals / Humans Idioma: En Revista: Science Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos