DNA-origami-directed virus capsid polymorphism.
Nat Nanotechnol
; 18(10): 1205-1212, 2023 10.
Article
em En
| MEDLINE
| ID: mdl-37460794
ABSTRACT
Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA-DNA origami structures to pave way for next-generation cargo protection and targeting strategies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Capsídeo
/
Nanoestruturas
Idioma:
En
Revista:
Nat Nanotechnol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Finlândia