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Elevated translationally controlled tumour protein promotes oral cancer progression and poor outcome.
Sharma, Dipti; Pawar, Sagar N; Sulkshane, Prasad; Waghole, Rohit; Yasser, Mohd; Pawar, Sushil S; Kannan, Sadhana; Chaudhary, Nazia; Kalwar, Anjali; Patil, Rahul; Nair, Sudhir; Dalal, Sorab N; Teni, Tanuja.
Afiliação
  • Sharma D; Teni Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Navi Mumbai, India.
  • Pawar SN; Homi Bhabha National Institute (HBNI), Mumbai, India.
  • Sulkshane P; Teni Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Navi Mumbai, India.
  • Waghole R; Teni Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Navi Mumbai, India.
  • Yasser M; Teni Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Navi Mumbai, India.
  • Pawar SS; Teni Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Navi Mumbai, India.
  • Kannan S; Department of Oral Pathology & Microbiology, KBH Dental College and Hospital, Nashik, India.
  • Chaudhary N; Clinical Research Secretariat, ACTREC, TMC, Navi Mumbai, India.
  • Kalwar A; Homi Bhabha National Institute (HBNI), Mumbai, India.
  • Patil R; Cell and Tumor Biology, ACTREC, TMC, Navi Mumbai, India.
  • Nair S; Teni Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Navi Mumbai, India.
  • Dalal SN; Department of Oral Pathology & Microbiology, KBH Dental College and Hospital, Nashik, India.
  • Teni T; Department of Surgical Oncology, Tata Memorial Hospital, Mumbai, India.
J Oral Pathol Med ; 52(9): 849-859, 2023 Oct.
Article em En | MEDLINE | ID: mdl-37573872
ABSTRACT

BACKGROUND:

Translationally controlled tumour protein (TCTP) is a multifunctional protein elevated in multiple cancers. However, studies on its role in oral carcinogenesis and prognosis are rare. We recently reported the role of its interacting partner, MCL1, in oral cancer progression and outcome. Hence, the present study aimed to assess TCTP expression in oral tumorigenesis and its association with patient outcomes alone and in combination with MCL1.

METHODS:

TCTP expression was assessed by immunohistochemistry and immunoblotting in oral tissues and cells, respectively. Cell viability post siRNA/dihydroartemisinin treatment was analysed by tetrazolium salt assay. Cell survival, invasion and tumorigenic potential post TCTP knockdown were assessed by clonogenic, Matrigel and soft-agar assays, respectively. The association of TCTP with patient outcome was analysed by Kaplan-Meier and Cox regression.

RESULTS:

TCTP was significantly overexpressed in oral premalignant lesions (p < 0.0001), oral tumours (p < 0.0001) and oral dysplastic and cancer cells versus normal oral mucosa and also in recurrent (p < 0.05) versus non-recurrent oral tumours. Further, elevated TCTP was significantly (p < 0.05) associated with poor recurrence free survival (RFS) and poor overall survival (OS; hazard ratio = 2.29; p < 0.05). Intriguingly, the high co-expression of TCTP and MCL1 further reduced the RFS (p < 0.05) and OS (p < 0.05; hazard-ratio = 3.49; p < 0.05). Additionally, TCTP knockdown decreased survival (p < 0.05), invasion (p < 0.01) and in vitro tumorigenic potential (p < 0.0001). Dihydroartemisinin treatment reduced TCTP levels and viability of oral cancer cells.

CONCLUSION:

Our studies demonstrate an oncogenic role of TCTP in oral cancer progression and poor outcome. Thus, TCTP may be a potential prognostic marker and therapeutic target in oral cancers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Contexto em Saúde: 1_ASSA2030 / 2_ODS3 Problema de saúde: 1_doencas_nao_transmissiveis / 2_muertes_prematuras_enfermedades_notrasmisibles Assunto principal: Neoplasias Bucais / Artemisininas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Oral Pathol Med Assunto da revista: ODONTOLOGIA / PATOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Contexto em Saúde: 1_ASSA2030 / 2_ODS3 Problema de saúde: 1_doencas_nao_transmissiveis / 2_muertes_prematuras_enfermedades_notrasmisibles Assunto principal: Neoplasias Bucais / Artemisininas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Oral Pathol Med Assunto da revista: ODONTOLOGIA / PATOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia
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