A 2,7-dichlorofluorescein derivative to monitor microcalcifications.

Tholen, Patrik; Brown, Connor N; Keil, Claudia; Bayir, Ali; Zeng, Hui-Hui; Haase, Hajo; Thompson, Richard B; Lengyel, Imre; Yücesan, Gündog

Tholen, Patrik; Brown, Connor N; Keil, Claudia; Bayir, Ali; Zeng, Hui-Hui; Haase, Hajo; Thompson, Richard B; Lengyel, Imre; Yücesan, Gündog.

Afiliação

Tholen P; Institute for Food Chemistry and Toxicology, Germany, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
Brown CN; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast BT9 7BL, UK.
Keil C; Institute for Food Chemistry and Toxicology, Germany, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
Bayir A; The Department of Chemistry, Yildiz Technical University, 34220, Esenler, Istanbul, Turkey.
Zeng HH; Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
Haase H; Institute for Food Chemistry and Toxicology, Germany, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
Thompson RB; Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
Lengyel I; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast BT9 7BL, UK.
Yücesan G; Institute for Food Chemistry and Toxicology, Germany, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.

Mol Syst Des Eng ; 7(11): 1415-1421, 2022 Nov 01.

Article em En | MEDLINE | ID: mdl-37927331

ABSTRACT

ABSTRACT

Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Mol Syst Des Eng Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha

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