Calcium flux alterations in erythrocytes from sickle cell mice: The relevance of mean corpuscular volume.
Blood Cells Mol Dis
; 104: 102800, 2024 01.
Article
em En
| MEDLINE
| ID: mdl-37951090
ABSTRACT
Red blood cells (RBC) from patients with sickle cell disease (SCD) have elevated calcium levels at baseline, which are further elevated upon deoxygenation. Here we examined baseline calcium levels and calcium flux in RBCs from a mouse model of SCD mice. We found that akin to humans with SCD, sickle (HbSS) Townes mice, have higher baseline levels and increased calcium flux in RBCs compared to control (HbAA) animals. As HbSS mice, unlike humans with SCD, have high mean corpuscular volume compared with HbAA, we highlight the importance of adjusting biochemical results to number of RBCs rather than hematocrit during the analysis and interpretation of the results. Our findings add to the face validity of humanized sickle cell mice and support its use for studies of RBC calcium flux in SCD.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Índices de Eritrócitos
/
Anemia Falciforme
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Blood Cells Mol Dis
/
Blood cells mol. dis
/
Blood cells, molecules & diseases
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos