Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus.
Stem Cells
; 42(3): 266-277, 2024 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-38066665
ABSTRACT
Adult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Músculo Esquelético
/
Células-Tronco Adultas
Limite:
Animals
Idioma:
En
Revista:
Stem Cells
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos