Secondary Structure Analysis of Fasciola from Semi-wild Ruminants of Northeast India.
Acta Parasitol
; 69(1): 599-608, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38261242
ABSTRACT
PURPOSE:
The objective of this study is to study the secondary structure analysis of Fasciola flukes from a rare mithun host from Manipur. Fascioliasis, a neglected tropical trematodiasis, is poorly studied in India and is widely believed to be predominantly caused by F. gigantica. Through this study, we want to assess the flukes from the rare semi-wild ruminants of Northeast India. This study is important as the mithun population is semi-wild and its population is declining in Manipur.METHODS:
Sample collected from the difficult and challenging terrain of Northeast India. The sample was collected from mithun and observed under the microscope. DNA was isolated, sequenced, and analyzed using various bioinformatics tools. The secondary structure analysis of the Internal Transcribed Spacer 2 (ITS2) region was also performed.RESULTS:
The secondary structure species tree corroborated the Bayesian inference and, hence, strengthened the phylogeny reconstructed. The annotated ITS2 sequence and RNA secondary of the Manipur isolate displayed the typical four-helix or four-domain model. Helix III reveals the presence of the UGGU motif with other deviations like UGG and GGU.CONCLUSION:
This is an in-depth analysis of the secondary structure of Fasciola species. The present study has demonstrated the usefulness of ITS2 and its secondary structures for characterizing parasites. The information on fascioliasis in the mithun's population presents itself useful with regards to their conservation strategy as their populations in both Manipur and Nagaland are dwindling.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_fascioliasis
/
3_neglected_diseases
Assunto principal:
Filogenia
/
Ruminantes
/
Fasciola
/
Fasciolíase
/
Conformação de Ácido Nucleico
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Acta Parasitol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Índia