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Biofabricated poly (γ-glutamic acid) bio-ink reinforced with calcium silicate exhibiting superior mechanical properties and biocompatibility for bone regeneration.
Chien, Ming-Hui; Chen, Cheng-Yu; Yeh, Chun-Liang; Huang, Hsin-Yi; Chou, Han-Yi; Chen, Yi-Wen; Lin, Chun-Pin.
Afiliação
  • Chien MH; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
  • Chen CY; x-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung, Taiwan.
  • Yeh CL; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
  • Huang HY; Graduate Institute of Dental Science and Oral Health Industries, China Medical University, Taichung, Taiwan.
  • Chou HY; Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
  • Chen YW; Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
  • Lin CP; Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan.
J Dent Sci ; 19(1): 479-491, 2024 Jan.
Article em En | MEDLINE | ID: mdl-38303841
ABSTRACT
Background/

purpose:

The modification in 3D hydrogels, tissue engineering, and biomaterials science has enabled us to fabricate novel substitutes for bone regeneration. This study aimed to combine different biomaterials by 3D technique to fabricate a promising all-rounded hydrogel for bone regeneration. Materials and

methods:

In this study, glycidyl methacrylate (GMA)-modified poly γ-glutamic acid (γ-PGA-GMA) hydrogels with calcium silicate (CS) hydrogel of different concentrations were fabricated by a 3D printing technique, and their biocompatibility and capability in bone regeneration were also evaluated.

Results:

The results showed that CS γ-PGA-GMA could be successfully fabricated, and the presence of CS enhanced the rheological and mechanical properties of γ-PGA-GMA hydrogels, thus making them more adept at 3D printing and implantations. SEM images of the surface structure showed that higher CS concentrations (5% and 10%) contributed to denser surface architectures, thus achieving improved cellular adhesion and stem cell proliferation. Furthermore, higher concentrations of CS resulted in elevated expressions of osteogenic-related markers such as alkaline phosphatase (ALP) and osteocalcin (OC), as well as enhanced calcium deposition represented by the increased Alizarin Red S staining. In vivo studies referring to critical defects of rabbit femur further showed that the existence of hydrogels alone was able to induce partial bone regeneration, demonstrated by the results from quantitative and qualitative analysis of micro-CT scans. However, CS alterations caused significant increases in bone regeneration, as indicated by micro-CT and histological staining.

Conclusion:

These results robustly suggest combining different biomaterials is crucial to producing a well-rounded hydrogel for tissue regeneration. We hope this study could be applied as a platform for others to brainstorm potential out-of-the-box solutions, contributing to developing high-potential biomaterials for bone regeneration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Qualitative_research Idioma: En Revista: J Dent Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Taiwan

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Qualitative_research Idioma: En Revista: J Dent Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Taiwan
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