Antioxidant and Antineoplastic Activities of Hibiscus sabdariffa Linn. Petal Extracts against Oral Squamous Cell Carcinoma Cell Line.
Oral Health Prev Dent
; 22: 131-138, 2024 02 20.
Article
em En
| MEDLINE
| ID: mdl-38376437
ABSTRACT
PURPOSE:
To assess the antioxidant and antineoplastic effects of Hibiscus sabdariffa Linn. on oral squamous cell carcinoma cells. MATERIALS ANDMETHODS:
Human squamous cell carcinoma HSCC cells were tested for cytotoxicity by a methanol extract of Hibiscus sabdariffa (MEHSP). After 24, 48, and 72 h, the MTT assay and Trypan blue exclusion test were used to determine cell survival and death. 2, 2-diphenyl-1-picrylhydrazyl (DPPH), DNA Protection Assay (DPA), and ferric reducing antioxidant power assay (FRAPA) measured the antioxidant activity of MEHSP.RESULTS:
The antioxidant activity (%) ranged from 47.92-82.24 in the DPPH test, 11.61-73.65 in the DPA, and 4.97-52.09 in the FRAPA. The HSCC in-vitro cytotoxicity assay showed dose- and time-dependent cell viability. MEHSP at 5 µg/ml inhibited viable cells, while increasing MEHSP doses decreased cell viability. The Trypan blue exclusion test showed that MEHSP significantly reduced cell viability at 24, 48, and 72 h.CONCLUSION:
Hibiscus sabdariffa contains antioxidant and HSCC-cytotoxic properties.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Problema de saúde:
6_larynx_cancer
/
6_mouth_oropharynx_cancers
Assunto principal:
Compostos de Bifenilo
/
Neoplasias Bucais
/
Carcinoma de Células Escamosas
/
Hibiscus
/
Neoplasias de Cabeça e Pescoço
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Oral Health Prev Dent
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article