Sister chromatid cohesion establishment during DNA replication termination.
Science
; 384(6691): 119-124, 2024 Apr 05.
Article
em En
| MEDLINE
| ID: mdl-38484038
ABSTRACT
Newly copied sister chromatids are tethered together by the cohesin complex, but how sister chromatid cohesion coordinates with DNA replication is poorly understood. Prevailing models suggest that cohesin complexes, bound to DNA before replication, remain behind the advancing replication fork to keep sister chromatids together. By visualizing single replication forks colliding with preloaded cohesin complexes, we find that the replisome instead pushes cohesin to where a converging replisome is met. Whereas the converging replisomes are removed during DNA replication termination, cohesin remains on nascent DNA and provides cohesion. Additionally, we show that CMG (CDC45-MCM2-7-GINS) helicase disassembly during replication termination is vital for proper cohesion in budding yeast. Together, our results support a model wherein sister chromatid cohesion is established during DNA replication termination.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Troca de Cromátide Irmã
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Cromátides
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Proteínas de Saccharomyces cerevisiae
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Replicação do DNA
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Coesinas
Idioma:
En
Revista:
Sci. (N.Y., N.Y.)
/
Science
Ano de publicação:
2024
Tipo de documento:
Article