Is myeloid-derived growth factor a ligand of the sphingosine-1-phosphate receptor 2?
Biochem Biophys Res Commun
; 706: 149766, 2024 04 30.
Article
em En
| MEDLINE
| ID: mdl-38484568
ABSTRACT
Secretory myeloid-derived growth factor (MYDGF) exerts beneficial effects on organ repair, probably via a plasma membrane receptor; however, the identity of the expected receptor has remained elusive. In a recent study, MYDGF was reported as an agonist of the sphingosine-1-phosphate receptor 2 (S1PR2), an A-class G protein-coupled receptor that mediates the functions of the signaling lipid, sphingosine-1-phosphate (S1P). In the present study, we conducted living cell-based functional assays to test whether S1PR2 is a receptor for MYDGF. In the NanoLuc Binary Technology (NanoBiT)-based ß-arrestin recruitment assay and the cAMP-response element (CRE)-controlled NanoLuc reporter assay, S1P could efficiently activate human S1PR2 overexpressed in human embryonic kidney (HEK) 293T cells; however, recombinant human MYDGF, overexpressed either from Escherichia coli or HEK293 cells, had no detectable effect. Thus, the results demonstrated that human MYDGF is not a ligand of human S1PR2. Considering the high conservation of MYDGF and S1PR2 in evolution, MYDGF is also probably not a ligand of S1PR2 in other vertebrates.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esfingosina
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Fator Estimulador de Colônias de Granulócitos
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Receptores de Lisoesfingolipídeo
Limite:
Animals
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Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
/
Biochem. biophys. res. commun
/
Biochemical and biophysical research communications
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China