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Proteomic study of left ventricle and cortex in rats after myocardial infarction.
Chang, Mengli; Wang, Huanhuan; Lei, Yuxin; Yang, Hongjun; Xu, Jing; Tang, Shihuan.
Afiliação
  • Chang M; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
  • Wang H; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
  • Lei Y; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
  • Yang H; Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
  • Xu J; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
  • Tang S; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. shtang@icmm.ac.cn.
Sci Rep ; 14(1): 6866, 2024 03 22.
Article em En | MEDLINE | ID: mdl-38514755
ABSTRACT
Myocardial infarction (MI) induces neuroinflammation indirectly, chronic neuroinflammation may cause neurodegenerative diseases. Changes in the proteomics of heart and brain tissue after MI may shed new light on the mechanisms involved in neuroinflammation. This study explored brain and heart protein changes after MI with a data-independent acquisition (DIA) mode proteomics approach. Permanent ligation of the left anterior descending coronary artery (LAD) was performed in the heart of rats, and the immunofluorescence of microglia in the brain cortex was performed at 1d, 3d, 5d, and 7d after MI to detect the neuroinflammation. Then proteomics was accomplished to obtain the vital proteins in the heart and brain post-MI. The results show that the number of microglia was significantly increased in the Model-1d group, the Model-3d group, the Model-5d group, and the Model-7d group compared to the Sham group. Various proteins were obtained through DIA proteomics. Linking to key targets of brain disease, 14 proteins were obtained in the brain cortex. Among them, elongation of very long chain fatty acids protein 5 (ELOVL5) and ATP-binding cassette subfamily G member 4 (ABCG4) were verified through western blotting (WB). The results of WB were consistent with the proteomics results. Therefore, these proteins may be related to the pathogenesis of neuroinflammation after MI.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ventrículos do Coração / Infarto do Miocárdio Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ventrículos do Coração / Infarto do Miocárdio Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
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