Comparative evaluation of trypsin and elastase digestion techniques for isolation of murine retinal vasculature.
Microvasc Res
; 154: 104682, 2024 07.
Article
em En
| MEDLINE
| ID: mdl-38521153
ABSTRACT
Dysfunctional pericytes and disruption of adherens or tight junctions are related to many microvascular diseases, including diabetic retinopathy. In this context, visualizing retinal vascular architecture becomes essential for understanding retinal vascular disease pathophysiology. Although flat mounts provide a demonstration of the retinal blood vasculature, they often lack a clear view of microaneurysms and capillary architecture. Trypsin and elastase digestion are the two techniques for isolating retinal vasculatures in rats, mice, and other animal models. Our observations in the present study reveal that trypsin digestion impacts the association between pericytes and endothelial cells. In contrast, elastase digestion effectively preserves these features in the blood vessels. Furthermore, trypsin digestion disrupts endothelial adherens and tight junctions that elastase digestion does not. Therefore, elastase digestion emerges as a superior technique for isolating retinal vessels, which can be utilized to collect reliable and consistent data to comprehend the pathophysiology of disorders involving microvascular structures.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasos Retinianos
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Elastase Pancreática
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Tripsina
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Pericitos
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Camundongos Endogâmicos C57BL
Limite:
Animals
Idioma:
En
Revista:
Microvasc Res
/
Microvasc. res
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Microvascular research
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos