Salt-Dependent Self-Association of Trinucleotide Repeat RNA Sequences.
J Phys Chem Lett
; 15(14): 3820-3827, 2024 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-38557079
ABSTRACT
Repeat RNA sequences self-associate to form condensates. Simulations of a coarse-grained single-interaction site model for (CAG)n (n = 30 and 31) show that the salt-dependent free energy gap, ΔGS, between the ground (perfect hairpin) and the excited state (slipped hairpin (SH) with one CAG overhang) of the monomer for (n even) is the primary factor that determines the rates and yield of self-assembly. For odd n, the free energy (GS) of the ground state, which is an SH, is used to predict the self-association kinetics. As the monovalent salt concentration, CS, increases, ΔGS and GS increase, which decreases the rates of dimer formation. In contrast, ΔGS for shuffled sequences, with the same length and sequence composition as (CAG)31, is larger, which suppresses their propensities to aggregate. Although demonstrated explicitly for (CAG) polymers, the finding of inverse correlation between the free energy gap and RNA aggregation is general.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
/
Repetições de Trinucleotídeos
Idioma:
En
Revista:
J Phys Chem Lett
/
J. phys. chem. lett
/
The journal of physical chemistry letters
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos