Application of HepaRG cells for genotoxicity assessment: a review.
J Environ Sci Health C Toxicol Carcinog
; 42(3): 214-237, 2024.
Article
em En
| MEDLINE
| ID: mdl-38566478
ABSTRACT
There has been growing interest in the use of human-derived metabolically competent cells for genotoxicity testing. The HepaRG cell line is considered one of the most promising cell models because it is TP53-proficient and retains many characteristics of primary human hepatocytes. In recent years, HepaRG cells, cultured in both a traditional two-dimensional (2D) format and as more advanced in-vivo-like 3D spheroids, have been employed in assays that measure different types of genetic toxicity endpoints, including DNA damage, mutations, and chromosomal damage. This review summarizes published studies that have used HepaRG cells for genotoxicity assessment, including cell model evaluation studies and risk assessment for various compounds. Both 2D and 3D HepaRG models can be adapted to several high-throughput genotoxicity assays, generating a large number of data points that facilitate quantitative benchmark concentration modeling. With further validation, HepaRG cells could serve as a unique, human-based new alternative methodology for in vitro genotoxicity testing.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Hepatócitos
/
Testes de Mutagenicidade
Limite:
Humans
Idioma:
En
Revista:
J Environ Sci Health C Toxicol Carcinog
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos