The single strand template shortening strategy improves the sensitivity and specificity of solid-state nanopore detection.
Chem Commun (Camb)
; 60(35): 4723-4726, 2024 Apr 25.
Article
em En
| MEDLINE
| ID: mdl-38597243
ABSTRACT
Through controlling the ssDNA product length of rolling circle amplification with AcyNTP, here we develop a nanopore signal enhancement strategy (STSS), which can successfully transfer the short oligonucleotide targets into long ssDNAs with appropriate lengths that can generate significant translocation currents. By labelling the RCA product with tags such as tetrahedral structures and isothermal amplicons, the resolution, signal specificity, and target range of the STSS can be further extended.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA de Cadeia Simples
/
Técnicas de Amplificação de Ácido Nucleico
/
Nanoporos
Idioma:
En
Revista:
Chem Commun (Camb)
Assunto da revista:
QUIMICA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China