Supermolecular Confined Silicon Phosphorescence Nanoprobes for Time-Resolved Hypoxic Imaging Analysis.
Anal Chem
; 96(16): 6467-6475, 2024 04 23.
Article
em En
| MEDLINE
| ID: mdl-38602368
ABSTRACT
Room temperature phosphorescence (RTP) nanoprobes play crucial roles in hypoxia imaging due to their high signal-to-background ratio (SBR) in the time domain. However, synthesizing RTP probes in aqueous media with a small size and high quantum yield remains challenging for intracellular hypoxic imaging up to present. Herein, aqueous RTP nanoprobes consisting of naphthalene anhydride derivatives, cucurbit[7]uril (CB[7]), and organosilicon are reported via supermolecular confined methods. Benefiting from the noncovalent confinement of CB[7] and hydrolysis reactions of organosilicon, such small-sized RTP nanoprobes (5-10 nm) exhibit inherent tunable phosphorescence (from 400 to 680 nm) with microsecond second lifetimes (up to â¼158.7 µs) and high quantum yield (up to â¼30%). The as-prepared RTP nanoprobes illustrate excellent intracellular hypoxia responsibility in a broad range from â¼0.1 to 21% oxygen concentrations. Compared to traditional fluorescence mode, the SBR value (â¼108.69) of microsecond-range time-resolved in vitro imaging is up to 2.26 times greater in severe hypoxia (<0.1% O2), offering opportunities for precision imaging analysis in a hypoxic environment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imidazolidinas
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Compostos Macrocíclicos
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Compostos Heterocíclicos com 2 Anéis
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Imidazóis
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
/
Anal. chem
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Analytical chemistry
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China