Comparative evaluation of liquid-liquid extraction and nanosorbent extraction for HPLC-PDA analysis of cabazitaxel from rat plasma.
J Pharm Biomed Anal
; 245: 116149, 2024 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-38678858
ABSTRACT
A precise, sensitive, accurate, and validated reverse-phase high-performance liquid chromatography (RP-HPLC) method with a bioanalytical approach was utilized to analyze Cabazitaxel (CBZ) in rat plasma. Comparative research on extraction recoveries was performed between traditional liquid-liquid extraction (LLE) and synthesized graphene oxide (GO) based magnetic solid phase extraction (GO@MSPE). The superparamagnetic hybrid nanosorbent was synthesized using the combination of iron oxide and GO and subsequently applied for extraction and bioanalytical quantification of CBZ from plasma by (HPLC-PDA) analysis. Fourier- transform infrared spectroscopy (FT-IR), particle size, scanning electron microscopy (SEM), and x-ray diffraction (XRD) analysis were employed in the characterization of synthesized GO@MSPE nanosorbent. The investigation was accomplished using a shim pack C18 column (150â¯mm×4.6â¯mm, 5⯵m) with a binary gradient mobile phase consisting of formic acid acetonitrile water (0.17525, v/v/v) at a 0.8â¯mL/min flow rate, and a λmax of 229â¯nm. The limits of detection (LOD) and quantitation (LOQ) have been determined to be 50 and 100â¯ng/mL for both LLE and SPE techniques. The linearity range of the approach encompassed from 100 to 5000â¯ng/mL and was found to be linear (coefficient of determination > 0.99) for CBZ. The proposed method showed extraction recovery of 76.8-88.4% for the synthesized GO@MSPE and 69.3-77.4% for LLE, suggesting that the proposed bioanalytical approach was robust and qualified for all validation parameters within the acceptable criteria. Furthermore, the developed hybrid GO@MSPE nanosorbent with the help of the proposed RP-HPLC method, showed a significant potential for the extraction of CBZ in bioanalysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extração em Fase Sólida
/
Limite de Detecção
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Extração Líquido-Líquido
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Grafite
Limite:
Animals
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2024
Tipo de documento:
Article