Evaluation of the utility and cost of secondary confirmatory testing for Neisseria gonorrhoeae identification from culture.
Diagn Microbiol Infect Dis
; 109(3): 116336, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38723452
ABSTRACT
Current guideline recommends the use of two identification methods for Neisseria gonorrhoeae. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) is now used for primary identification and may be sufficient for definitive identification of N. gonorrhoeae. The performance of three secondary tests (BactiCard, RapID NH and NET test) were compared using 45 bacterial isolates, including 37 Neisseria species. These secondary tests demonstrated diminished specificity (67% - 88%) for N. gonorrhoeae compared with MALDI-TOF. Additionally, data from six clinical microbiology laboratories was used to compare confirmatory test costs and the agreement of results with MALDI-TOF. Discrepancies were documented for 9.4% of isolates, though all isolates (n= 288) identified by MALDI-TOF as N. gonorrhoeae were confirmed by the reference laboratory. These data demonstrate that MALDI-TOF alone is sufficient for N. gonorrhoeae identification, as secondary did not add diagnostic value but do add costs to the testing process.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gonorreia
/
Sensibilidade e Especificidade
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Neisseria gonorrhoeae
Limite:
Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
/
Diagn. microbiol. infect. dis
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Diagnostic microbiology and infectious disease
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Canadá