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Extraction and Isolation of Two Polysaccharides from Chloranthus japonicus Sieb. and Evaluation of Their Anti-Gastric Cancer Activities.
Liu, Ju; Li, Wenfeng; Jin, Lu; Wang, Yingchao; Xu, Xinjun; Ma, Enyao; Yang, Depo; Zhao, Zhimin.
Afiliação
  • Liu J; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
  • Li W; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
  • Jin L; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
  • Wang Y; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
  • Xu X; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
  • Ma E; Department of Drug Discovery, Guangzhou Caizhilin Pharmaceutical Co., Ltd., Guangzhou 510360, China.
  • Yang D; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
  • Zhao Z; School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Molecules ; 29(9)2024 Apr 29.
Article em En | MEDLINE | ID: mdl-38731534
ABSTRACT
Two unreported heteropolysaccharides, denoted as YCJP-1 and YCJP-2, were isolated from the herbs of Chloranthus japonicus. YCJP-1 was a heteropolysaccharide composed of glucose, galactose, arabinose, mannose, rhamnose, and a minor proportion of uronic acids, with the molecular weight mainly distributed in the 74,475-228,443 Da range. YCJP-2 was mainly composed of glucose, mannose, and galactose, with the molecular weights ranging from 848 to 5810 Da. To further evaluate the anti-gastric cancer effects of C. japonicus, the inhibitory effects of the crude polysaccharide (YCJP) and the purified polysaccharides (YCJP-1 and YCJP-2) were determined using a CCK-8 assay and colon-forming assay on MGC-803 and AGS gastric cancer cell lines. Our results showed that YCJP, YCJP-1, and YCJP-2 possess prominent inhibitory effects on the proliferation of MGC-803 and AGS cells, and the AGS cell was more sensitive to YCJP, YCJP-1, and YCJP-2. Moreover, YCJP-2 demonstrated superior anti-gastric cancer effects compared to YCJP-1. This could potentially be attributed to YCJP-2's higher glucose content and narrower molecular weight distribution.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos / Neoplasias Gástricas / Proliferação de Células Limite: Humans Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos / Neoplasias Gástricas / Proliferação de Células Limite: Humans Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
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