Sensitive colorimetric detection of Vibrio vulnificus based on target-induced shielding against the peroxidase-mimicking activity of CeO2@PtRu nanozyme.
Food Chem
; 454: 139757, 2024 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-38805924
ABSTRACT
Vibrio vulnificus infection caused by contaminated aquatic products and seawater can lead to severe disease and high mortality. The development of a rapid and sensitive detection method for Vibrio vulnificus is vital to effectively prevent infection in advance. In this study, CeO2@PtRu with high peroxidase activity was used to construct a colorimetric immunoassay for Vibrio vulnificus detection by conjugating polyclonal antibodies via the biotin-streptavidin system. The developed colorimetric biosensor for Vibrio vulnificus demonstrated rapid operability and good sensitivity with a detection range from 104 CFU/mL to 109 CFU/mL, and the limit of detection (LOD) is 193 CFU/mL. Moreover, the colorimetric biosensor showed excellent specificity and good recoveries from 98.70% to 102.10% with RSD < 7.45% for spiked real samples. This novel CeO2@PtRu-based colorimetric biosensor has great application potential for the sensitive detection of Vibrio vulnificus in seafood.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Cério
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Alimentos Marinhos
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Colorimetria
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Vibrio vulnificus
Limite:
Animals
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2024
Tipo de documento:
Article