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Amplification-free detection of Escherichia coli using an acidic deoxyribozyme-based paper device.
Zhang, Guangxiao; Wu, Yunping; Xue, Wei; Wang, Dong; Chang, Yangyang; Liu, Meng.
Afiliação
  • Zhang G; School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China.
  • Wu Y; Dalian POCT laboratory, Dalian, 116024, China. yychang@dlut.edu.cn.
  • Xue W; School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China.
  • Wang D; Dalian POCT laboratory, Dalian, 116024, China. yychang@dlut.edu.cn.
  • Chang Y; School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China.
  • Liu M; Dalian POCT laboratory, Dalian, 116024, China. yychang@dlut.edu.cn.
Chem Commun (Camb) ; 60(53): 6741-6744, 2024 Jun 27.
Article em En | MEDLINE | ID: mdl-38809259
ABSTRACT
We reported a colorimetric paper-based device by integrating the modified acid RNA-cleaving DNAzymes (MaRCD-EC1) for highly sensitive (detection limit = 102 CFU mL-1), and rapid (within 30 min) detection of E. coli without amplification. This device exhibited a clinical sensitivity of 100% and a specificity of 100% in identifying E. coli-associated urinary tract infections (UTIs) using the clinical urine samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papel / Colorimetria / DNA Catalítico / Escherichia coli Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papel / Colorimetria / DNA Catalítico / Escherichia coli Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China