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Development of an enzyme-linked immunosorbent assay using a monoclonal antibody to a dominant epitope in non-structural protein 4 of porcine reproductive and respiratory syndrome virus.
Fu, Chaolun; Shao, Qingyuan; Zhang, Lei; Cui, Xinyu; Chen, Ting; Tian, Chang; Qian, Fenglu; Chu, Xuefei; Li, Yingchao; Yang, Pingping; Hou, Yanmeng; Xiao, Yihong.
Afiliação
  • Fu C; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Shao Q; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Zhang L; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Cui X; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Chen T; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Tian C; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Qian F; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Chu X; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Li Y; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Yang P; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China.
  • Hou Y; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China. Electronic address: lemonhym@163.com.
  • Xiao Y; Department of Fundamental Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, PR China. Electronic address: xiaoyihong01@163.co
J Immunol Methods ; 530: 113697, 2024 Jul.
Article em En | MEDLINE | ID: mdl-38823576
ABSTRACT
Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is one of the most severe swine diseases causing great economic losses for the international swine industry. Non-structural protein 4 (NSP4) is critical to the life cycle of PRRSV and contains dominant B cell epitopes. This study prepared a monoclonal antibody against Nsp4, and 2D11, which contained the sequence 138KQGGGIVTRPSGQFCN153, was confirmed as the epitope. A 2D11-based double antibody sandwich enzyme-linked immunosorbent assay (dasELISA) was next developed with a cut value of 0.1987. A total of 1354 pig serum samples were detected by dasELISA and compared to a commercial ELISA kit (N-coated iELISA), resulting in a positive coincidence rate of 98.8% and negative coincidence rate of 96.9%. A total of 119 sera were positive by dasELISA while negative by iELISA. Higher positive rates by dasELISA were found in pig farms where PRRSV antibody levels varied widely. These results indicated that the dasELISA was a useful tool to detect PRRSV antibody in clinical samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Proteínas não Estruturais Virais / Vírus da Síndrome Respiratória e Reprodutiva Suína / Síndrome Respiratória e Reprodutiva Suína / Anticorpos Monoclonais / Anticorpos Antivirais Limite: Animals Idioma: En Revista: J Immunol Methods Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Proteínas não Estruturais Virais / Vírus da Síndrome Respiratória e Reprodutiva Suína / Síndrome Respiratória e Reprodutiva Suína / Anticorpos Monoclonais / Anticorpos Antivirais Limite: Animals Idioma: En Revista: J Immunol Methods Ano de publicação: 2024 Tipo de documento: Article
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