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MultiPrime: A reliable and efficient tool for targeted next-generation sequencing.
Xia, Han; Zhang, Zhe; Luo, Chen; Wei, Kangfei; Li, Xuming; Mu, Xiyu; Duan, Meilin; Zhu, Chuanlong; Jin, Luyi; He, Xiaoqing; Tang, Lingjie; Hu, Long; Guan, Yuanlin; Lam, David C C; Yang, Junbo.
Afiliação
  • Xia H; School of Automation Science and Engineering, Faculty of Electronic and Information Engineering Xi'an Jiaotong University Xi'an China.
  • Zhang Z; MOE Key Lab for Intelligent Networks & Networks Security, Faculty of Electronic and Information Engineering Xi'an Jiaotong University Xi'an China.
  • Luo C; Department of Research and Development Hugobiotech Beijing China.
  • Wei K; Department of Mechanical and Aerospace Engineering The Hong Kong University of Science and Technology Hong Kong China.
  • Li X; Department of Research and Development Hugobiotech Beijing China.
  • Mu X; Department of Research and Development Hugobiotech Beijing China.
  • Duan M; Department of Research and Development Hugobiotech Beijing China.
  • Zhu C; Department of Research and Development Hugobiotech Beijing China.
  • Jin L; Department of Research and Development Hugobiotech Beijing China.
  • He X; Department of Research and Development Hugobiotech Beijing China.
  • Tang L; Department of Research and Development Hugobiotech Beijing China.
  • Hu L; Department of Research and Development Hugobiotech Beijing China.
  • Guan Y; Department of Research and Development Hugobiotech Beijing China.
  • Lam DCC; Department of Research and Development Hugobiotech Beijing China.
  • Yang J; Department of Research and Development Hugobiotech Beijing China.
Imeta ; 2(4): e143, 2023 Nov.
Article em En | MEDLINE | ID: mdl-38868227
ABSTRACT
We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next-generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime-designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Imeta Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Imeta Ano de publicação: 2023 Tipo de documento: Article
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