YTHDF1 promotes the osteolytic bone metastasis of breast cancer via inducing EZH2 and CDH11 translation.
Cancer Lett
; 597: 217047, 2024 Aug 10.
Article
em En
| MEDLINE
| ID: mdl-38871245
ABSTRACT
Bone metastasis is common in breast cancer and more effective therapies are required, however, its molecular mechanism is poorly understood. Additionally, the role of the m6A reader YTHDF1 in bone metastasis of breast cancer has not been reported. Here, we reveal that the increased expression of YTHDF1 is clinically correlated with breast cancer bone metastases. YTHDF1 promotes migration, invasion, and osteoblast adhesion and induces osteoclast differentiation of cancer cells in vitro and vivo. Mechanically, RNA-seq, MeRIP-seq and RIP-seq analysis, and molecular biology experiments demonstrate that YTHDF1 translationally enhances EZH2 and CDH11 expression by reading m6A-enriched sites of their transcripts. Moreover, adeno-associated virus (AAV) was used to deliver shYTHDF1 (shYTHDF1-AAV) in intratibial injection models, eliciting a significant suppressive effect on breast cancer bone metastatic formation and osteolytic destruction. Overall, we uncovered that YTHDF1 promotes osteolytic bone metastases of breast cancer by inducing EZH2 and CDH11 translation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteólise
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Neoplasias Ósseas
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Neoplasias da Mama
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Caderinas
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Proteínas de Ligação a RNA
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Proteína Potenciadora do Homólogo 2 de Zeste
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Cancer Lett
/
Cancer lett
/
Cancer letters
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China