RNA Sequencing Screens the Key Genes and Pathways in a Mouse Model of HFpEF.
J Vasc Res
; 61(4): 166-178, 2024.
Article
em En
| MEDLINE
| ID: mdl-38880090
ABSTRACT
INTRODUCTION:
Heart failure with preserved ejection fraction (HFpEF) is a common syndrome with high morbidity and mortality but without available evidence-based therapies. It is essential to investigate changes in gene expression profiles in preclinical HFpEF animal models, with the aim of searching for novel therapeutic targets.METHODS:
Wild-type male C57BL/6J mice were administrated with a combination of high-fat diet (HFD) and inhibition of constitutive nitric oxide synthase using N-nitro-RESULTS:
A total of 1,347 genes were differentially expressed in the heart at week 5 and 7 post-intervention. Gene Ontology enrichment analysis indicated that these greatly changed genes were involved mainly in cell adhesion, neutrophil chemotaxis, cell communication, and other functions. Using hierarchical cluster analysis, these differentially expressed genes were classified into 16 profiles. Of these, three significant profiles were ultimately identified. Gene co-expression network analysis suggested troponin T type 1 (Tnnt1) directly regulated 31 neighboring genes and was considered to be at the core of the associated gene network.CONCLUSION:
The combined application of RNA sequencing, hierarchical cluster analysis, and gene network analysis identified Tnnt1 as the most important gene in the development of HFpEF.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Volume Sistólico
/
Função Ventricular Esquerda
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Perfilação da Expressão Gênica
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Modelos Animais de Doenças
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Redes Reguladoras de Genes
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Transcriptoma
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Insuficiência Cardíaca
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Camundongos Endogâmicos C57BL
Limite:
Animals
Idioma:
En
Revista:
J Vasc Res
Assunto da revista:
ANGIOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article