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Mass spectrometry-based metabolomics study of nicotine exposure in THP-1 monocytes.
Uhlig, Silvio; Olderbø, Bergitte Pearl; Samuelsen, Jan Tore; Uvsløkk, Solveig; Ivanova, Lada; Vanderstraeten, Camille; Grutle, Lene Aiko; Rangel-Huerta, Oscar Daniel.
Afiliação
  • Uhlig S; Nordic Institute of Dental Materials, Sognsveien 70A, 0855, Oslo, Norway. silvio.uhlig@niom.no.
  • Olderbø BP; Nordic Institute of Dental Materials, Sognsveien 70A, 0855, Oslo, Norway.
  • Samuelsen JT; Nordic Institute of Dental Materials, Sognsveien 70A, 0855, Oslo, Norway.
  • Uvsløkk S; Nordic Institute of Dental Materials, Sognsveien 70A, 0855, Oslo, Norway.
  • Ivanova L; Toxinology Research Group, Norwegian Veterinary Institute, P.O. Box 64, 1431, Ås, Norway.
  • Vanderstraeten C; Nordic Institute of Dental Materials, Sognsveien 70A, 0855, Oslo, Norway.
  • Grutle LA; Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000, Gent, Belgium.
  • Rangel-Huerta OD; Nordic Institute of Dental Materials, Sognsveien 70A, 0855, Oslo, Norway.
Sci Rep ; 14(1): 14957, 2024 06 28.
Article em En | MEDLINE | ID: mdl-38942832
ABSTRACT
The tobacco alkaloid nicotine is known for its activation of neuronal nicotinic acetylcholine receptors. Nicotine is consumed in different ways such as through conventional smoking, e-cigarettes, snuff or nicotine pouches. The use of snuff has been associated with several adverse health effects, such as inflammatory reactions of the oral mucosa and oral cavity cancer. We performed a metabolomic analysis of nicotine-exposed THP-1 human monocytes. Cells were exposed to 5 mM of the alkaloid for up to 4 h, and cell extracts and medium subjected to untargeted liquid chromatography high-resolution mass spectrometry. Raw data processing revealed 17 nicotine biotransformation products. Among these, cotinine and nornicotine were identified as the two major cellular biotransformation products. The application of multi- and univariate statistical analyses resulted in the annotation, up to a certain level of identification, of 12 compounds in the cell extracts and 13 compounds in the medium that were altered by nicotine exposure. Of these, four were verified as methylthioadenosine, cytosine, uric acid, and L-glutamate. Methylthioadenosine levels were affected in both cells and the medium, while cytosine, uric acid, and L-glutamate levels were affected in the medium only. The effects of smoking on the pathways involving these metabolites have been previously demonstrated in humans. Most of the other discriminating compounds, which were merely tentatively or not fully identified, were amino acids or amino acid derivatives. In conclusion, our preliminary data suggest that some of the potentially adverse effects related to smoking may also be expected when nicotine is consumed via snuff or nicotine pouches.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Monócitos / Metabolômica / Nicotina Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Noruega

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Monócitos / Metabolômica / Nicotina Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Noruega
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