Chitinophaga pollutisoli sp. nov., isolated from contaminated sediment.
Int J Syst Evol Microbiol
; 74(7)2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38963413
ABSTRACT
A Gram-stain-negative, yellow-pigmented, and facultatively aerobic bacterium, designated strain GPA1T, was isolated from plastic waste landfill soil in the Republic of Korea. The cells were non-motile short rods exhibiting oxidase-negative and catalase-positive activities. Growth was observed at 15-40â°C (optimum, 30â°C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-2.5â% (w/v) NaCl (optimum, 0â%). Menaquinone-7 was the sole respiratory quinone, and iso-C15ââ0, C16ââ1 ω5c, and iso-C17ââ0 3-OH were the major cellular fatty acids (>10â% of the total fatty acids). Phosphatidylethanolamine was identified as a major polar lipid. Phylogenetic analyses based on 16S rRNA gene sequences and 120 concatenated marker protein sequences revealed that strain GPA1T formed a distinct lineage within the genus Chitinophaga. The genome of strain GPA1T was 6078 kb in size with 53.8 mol% G+C content. Strain GPA1T exhibited the highest similarity to Chitinophaga rhizosphaerae T16R-86T, with a 98.6â% 16S rRNA gene sequence similarity, but their average nucleotide identity and digital DNA-DNA hybridization values were 82.5 and 25.9â%, respectively. Based on its phenotypic, chemotaxonomic, and phylogenetic characteristics, strain GPA1T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga pollutisoli sp. nov. is proposed. The type strain is GPA1T (=KACC 23415T=JCM 36644T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidiletanolaminas
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Filogenia
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Microbiologia do Solo
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Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Análise de Sequência de DNA
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Sedimentos Geológicos
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Vitamina K 2
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article