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Engineered Chlorella vulgaris improves bioethanol production and promises prebiotic application.
Saha, Sumedha; Maji, Sachin; Ghosh, Sudip K; Maiti, Mrinal K.
Afiliação
  • Saha S; Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
  • Maji S; Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
  • Ghosh SK; Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
  • Maiti MK; Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India. mrinalkmaiti@gmail.com.
World J Microbiol Biotechnol ; 40(9): 271, 2024 Jul 20.
Article em En | MEDLINE | ID: mdl-39030369
ABSTRACT
Microalgal biomass for biofuel production, integration into functional food, and feed supplementation has generated substantial interest worldwide due to its high growth rate, non-competitiveness for agronomic land, ease of cultivation in containments, and presence of several bioactive molecules. In this study, genetic engineering tools were employed to develop transgenic lines of freshwater microalga Chlorella vulgaris with a higher starch content, by up-regulating ADP-glucose pyrophosphorylase (AGPase), which is a rate-limiting enzyme in starch biosynthesis. Expression of the Escherichia coli glgC (AGPase homolog) gene in C. vulgaris led to an increase in total carbohydrate content up to 45.1% (dry cell weight, DCW) in the transgenic line as compared to 34.2% (DCW) in the untransformed control. The starch content improved up to 16% (DCW) in the transgenic alga compared to 10% (DCW) in the control. However, the content of total lipid, carotenoid, and chlorophyll decreased differentially in the transgenic lines. The carbohydrate-rich biomass from the transgenic algal line was used to produce bioethanol via yeast fermentation, which resulted in a higher ethanol yield of 82.82 mg/L as compared to 54.41 mg/L from the untransformed control. The in vitro digestibility of the transgenic algal starch revealed a resistant starch content of up to 7% of total starch. Faster growth of four probiotic bacterial species along with a lowering of the pH of the growth medium indicated transgenic alga to exert a positive prebiotic effect. Taken together, the study documents the utilization of genetically engineered C. vulgaris with enriched carbohydrates as bioethanol feedstock and functional food ingredients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Amido / Biomassa / Chlorella vulgaris / Etanol / Escherichia coli / Glucose-1-Fosfato Adenililtransferase / Prebióticos / Biocombustíveis / Microalgas / Fermentação Idioma: En Revista: World J Microbiol Biotechnol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Amido / Biomassa / Chlorella vulgaris / Etanol / Escherichia coli / Glucose-1-Fosfato Adenililtransferase / Prebióticos / Biocombustíveis / Microalgas / Fermentação Idioma: En Revista: World J Microbiol Biotechnol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Índia
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