CGRP promotes osteogenic differentiation by regulating macrophage M2 polarization through HDAC6/AKAP12 signaling pathway.
Regen Med
; 19(7-8): 379-391, 2024.
Article
em En
| MEDLINE
| ID: mdl-39072399
ABSTRACT
Aim:
To determine the mechanism of Calcitonin gene-related peptide (CGRP) in bone healing.Materials &methods:
Alkaline phosphatase (ALP) activity and inflammatory-factor levels were detected using ELISA. Osteogenic differentiation was assessed using Alizarin red staining technique. The interaction between histone deacetylase 6 (HDAC6) and A-kinase anchoring protein 12 (AKAP12) was investigated through Co- immunoprecipitation.Results:
CGRP treatment promoted rat bone marrow-derived macrophages (BMDMs) M2 polarization. CGRP facilitated osteogenic differentiation by enhancing M2 polarization of BMDMs. Mechanistically, CGRP promoted AKAP12 acetylation to activate the extracellular regulated protein kinases pathway by HDAC6 inhibition.Conclusion:
CGRP promoted M2 polarization of rat BMDMs and facilitated osteogenic differentiation through the HDAC6/AKAP12/extracellular regulated protein kinases signaling pathway, thereby promoting bone healing.
[Box see text].
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Transdução de Sinais
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Peptídeo Relacionado com Gene de Calcitonina
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Diferenciação Celular
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Proteínas de Ancoragem à Quinase A
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Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Regen Med
/
Regenerative medicine (Online)
Ano de publicação:
2024
Tipo de documento:
Article