Multiplexed CRISPR-Cas system targeting ASFV genes in vivo: solution lies within.
Microbiol Spectr
; 12(9): e0071424, 2024 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-39109857
ABSTRACT
The emergence and spread of the African swine fever virus (ASFV) posed a significant threat to the global swine breeding industry, calling for innovative approaches benefiting viral containment and control. A recent study (Z. Zheng, L. Xu, H. Dou, Y. Zhou, X., et al., Microbiol Spectr 12 e02164-23, 2024, https//doi.org/10.1128/spectrum.02164-23) established a multiplexed CRISPR-Cas system targeting the genome of ASFV and tested the consequent antiviral activity both in vitro and in vivo. Application of this system showed a significant reduction of viral replication in vitro, while the germline-edited pigs expressing this system exhibited normal growth with continuous guide RNA expression. Although no survival advantage was observed upon ASFV challenge compared with nonengineered pigs, this marks the first attempt of germline editing to pursue ASFV resistance and paves the way for future disease-resistant animal breeding approaches utilizing CRISPR-Cas technology.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Febre Suína Africana
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Vírus da Febre Suína Africana
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Sistemas CRISPR-Cas
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Edição de Genes
Limite:
Animals
Idioma:
En
Revista:
Microbiol Spectr
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China