Protocol for genetic engineering in Drosophila suzukii using microinjection.
STAR Protoc
; 5(3): 103248, 2024 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-39146186
ABSTRACT
The spotted wing Drosophila (Drosophila suzukii Matsumura) is recognized globally as a significant economic pest. Here, we present a protocol for genetic engineering in D. suzukii using microinjection. We describe steps for genetic engineering techniques, including transposon-mediated germline transformation, recombinase-mediated genome targeting, and CRISPR-mediated gene editing. This protocol can significantly expand the toolkit for functional genomics and genetic control studies of this pest. For complete details on the use and execution of this protocol, please refer to Schetelig and Handler,1 Schetelig et al.2 Yan et al.,3 and Yan et al.4.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Drosophila
/
Edição de Genes
/
Microinjeções
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
/
STAR protocols
Ano de publicação:
2024
Tipo de documento:
Article