A continuous fluorescence assay to measure nicotianamine synthase activity.
Methods Enzymol
; 702: 51-74, 2024.
Article
em En
| MEDLINE
| ID: mdl-39155120
ABSTRACT
S-adenosylmethionine (SAM) is most widely known as the biological methylating agent of methyltransferases and for generation of radicals by the iron-sulfur dependent Radical SAM enzymes. SAM also serves as a substrate in biosynthetic reactions that harvest the aminobutyrate moiety of the methionine, producing methylthioadenosine as a co-product. These reactions are found in the production of polyamines such as spermine, siderophores derived from nicotianamine, and opine metallophores staphylopine and pseudopaline, among others. This procedure defines a highly sensitive, continuous fluorescence assay for the determination of steady state kinetic parameters for enzymes that generate the co-product methylthioadenosine.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
S-Adenosilmetionina
/
Ensaios Enzimáticos
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2024
Tipo de documento:
Article