Scavenging of reactive oxygen species in Candidatus Brocadia fulgida through nanocompartments.

ABSTRACT

The antioxidant defense mechanisms for anaerobic ammonia oxidation (anammox) bacteria are still unclear. In this study, the potential antioxidant ability of nanocompartments in Candidatus Brocadia fulgida to typical reactive oxygen species (ROS) was investigated. The results showed that the copies of genes involved in anammox central metabolism were inhibited with hydrogen peroxide (H2O2), while the genes encoded putative anti-oxidative protein (nanocompartments and cargo HAO) up-regulated. The genetically engineered bacteria grew better and maintained the lower ROS levels (65.60 %-78.07 %) and higher electron transport activities (∼5-21 times) than the wild bacteria under H2O2 stimulus. Molecular docking confirmed that nanocompartment proteins could provide diverse sites to bind with H2O2 based on heme as the redox center. Additionally, the nanocompartments induced up-regulation of multiple protective pathways for coping with oxidative stress from H2O2, including antioxidant enzymes and other non-enzymatic pathways. Thus, the heme-containing nanocompartments presented great potential in preventing and relieving oxidative stress.