Glycated walnut meal peptidecalcium chelates (COS-MMGGED-Ca): Preparation, characterization, and calcium absorption-promoting.
Food Chem
; 462: 140975, 2025 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-39197240
ABSTRACT
This study isolated a novel peptide MMGGED with strong calcium-binding capacity from defatted walnut meal and synthesized a novel peptidecalcium chelate COS-MMGGED-Ca with high stability via glycation. Structural characterization and computer simulation identified binding sites, while in vitro digestion stability and calcium transport experiments explored the chelate's properties. Results showed that after glycation, COS-MMGGED bound Ca2+ with 88.75 ± 1.75 %, mainly via aspartic and glutamic acids. COS-MMGGED-Ca released Ca2+ steadily (60.27 %), with thermal denaturation temperature increased by 18 °C and 37 °C compared to MMGGED-Ca, indicating good processing performance. Furthermore, COS-MMGGED significantly enhanced Ca2+ transport across Caco-2 monolayers, 1.13-fold and 1.62-fold higher than CaCl2 and MMGGED, respectively, at 240 h. These findings prove glycation enhances structural properties, stability, calcium loading, and transport of peptidecalcium chelates, providing a scientific basis for developing novel efficient calcium supplements and high-value utilization of walnut meal.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Cálcio
/
Juglans
Limite:
Humans
Idioma:
En
Revista:
Food Chem
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Food chem
/
Food chemistry
Ano de publicação:
2025
Tipo de documento:
Article
País de afiliação:
China