[Size and organization of repetitive sequences in pigeon genome]. / Razmer i organizatsiia povtoriaiushchikhsia posledovatel'nostei v genome golubia.

ABSTRACT

Organization of sequences in pigeon genome and the spectrum of their repetition frequencies were studied by means of DNA/DNA reassociation. Reassociation of 125I-labelled DNA fractions isolated from pigeon total DNA attested the presence of rare repetitions with an average of 50 copies within a gaploid genome. The disposition of repetitive and unique sequences was studied by reassociation of the labelled fragments of different length with an essential excess of short fragments of an unlabelled DNA. Additional evidence was provided by estimation of hyperchromicity and resistance to nuclease S1 of long DNA fragments, reassociated to the given C0t values. It was demonstrated that approximately one fourth of the pigeon genome consists of intermittent repetitive and unique sequences with individual elements of average length of 2 and 37 kb, respectively (1 kb = = 1000 nucleotide base pairs). A hypothetical organization of palindromic sequences in pigeon genome is discussed in terms of the dependence of the value of zero binding to hydroxyapatite on the fragment length.