Immune response to bovine viral diarrhea virus induced by anti-idiotypic antibodies.
Clin Diagn Lab Immunol
; 1(1): 95-8, 1994 Jan.
Article
em En
| MEDLINE
| ID: mdl-7496930
ABSTRACT
We have previously prepared rabbit anti-idiotypic antibodies (anti-Ids) against the neutralizing monoclonal antibodies (MAbs) specific for the gp53 of bovine viral diarrhea virus (BVDV). The anti-Ids, purified by sequential immunoaffinity chromatography, inhibited the immunizing MAb from binding to the original antigens and blocked BVDV infection of cell cultures. This study evaluated immune responses in mice to the purified anti-Id reagents. BVDV-specific neutralizing antibodies were induced by the anti-Ids. The antisera (Ab3) induced by the anti-Ids immunoprecipitated gp53 from BVDV-infected Madin-Darby bovine kidney (MDBK) cell lysates. However, lymphocyte-proliferative responses were specific only for the respective immunizing antigens. These results suggest that the anti-Ids may bear an internal image of the gp53 to stimulate production of antibody but not to stimulate a virus-specific cellular immune response in mice.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Anti-Idiotípicos
/
Vírus da Diarreia Viral Bovina
/
Anticorpos Monoclonais
/
Anticorpos Antivirais
Limite:
Animals
Idioma:
En
Revista:
Clin Diagn Lab Immunol
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Estados Unidos