Induction of chromatid breaks and tetraploidy in Chinese hamster ovary cells by treatment with sodium arsenite during the G2 phase.
Mutat Res
; 319(2): 135-42, 1993 Oct.
Article
em En
| MEDLINE
| ID: mdl-7692289
ABSTRACT
Treatment of Chinese hamster ovary (CHO) cells with sodium arsenite during the G2 phase induced poorly condensed chromosomes and chromatid breaks. The induction of chromatid breaks was confirmed by the appearance of micronucleated cells after arsenite-treated G2 cells were allowed to re-enter interphase. When the duration of the G2 phase was artificially divided into 4 periods, more chromatid breaks were induced by treatment with arsenite during the very early G2 phase (or G2/S boundary). In addition to the induction of chromatid breaks, arsenite treatment also remarkably retarded the re-entry of mitotic cells into interphase. By replating and incubating arsenite-treated G2 cells in drug-free medium, we subsequently observed the appearance of a population of cells whose DNA content was between 4C and 8C, and metaphase cells with near-tetraploid chromosome numbers in the next mitotic division.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliploidia
/
Dano ao DNA
/
Carcinógenos
/
Cromátides
/
Compostos de Sódio
/
Arsenitos
Limite:
Animals
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Taiwan