Production of a functional full-length Xenopus laevis c-Myc protein in insect cells.
Gene
; 150(2): 325-30, 1994 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-7821800
ABSTRACT
C-Myc is a nuclear phosphoprotein whose normal cellular function has not yet been clearly defined. Studies with this protein have always been constrained by the difficulty of obtaining full-length c-Myc in an active form, whatever the expression system used. We report here experimental conditions optimized to increase the solubility and the purification of c-Myc in a baculovirus expression system. Such conditions allow the production of both soluble and active full-length c-Myc. Interestingly, soluble c-Myc is found associated with a 500-kDa high-molecular-mass complex comparable to that found in human and Xenopus laevis embryos, and which may be required for its function in vivo.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Proto-Oncogênicas c-myc
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França