[Recombinant murine cell lines, transformed by various vectors based on bovine type 1 papillomavirus and expressing human tissue plasminogen activator. II. Analysis of cell lines, producing recombinant tissue plasminogen activator]. / Rekombinantnye linii kletok myshi, transformirovannye raslichnymi vektorami na osnove bych'ego papillomavirusa tipa 1 i ékspressiruiushchie tkanevoi aktivator plazminogena cheloveka. II. Analiz kletochnykh linii, produtsiruiushchikh rekombinantnyi tkanevoi aktivator plazminogena.

ABSTRACT

We have characterized a number of recombinant cell lines established with BPV1 and Lx1 (containing duplication of LCR-E6-E7 sequence) vectors on the basis of C127 cells. It had been shown that Lx1 based vectors possess the higher number of intracellular copies than analogous vectors on the basis of wtBPV, and most part of them is integrated into the host genome. Using various concentrations of heavy metal salts we have developed the optimized procedure for induction of recombinant tPA synthesis which is controlled by the mouse MT1 promoter. A 8-fold increase of rtPA concentration was reached in the course of induction. It had been shown that native and non-glucosylated forms of recombinant and human tPA are identical in their properties.